LC*GC
June 2013
May 2013
April 2013
March 2013
February 2013
January 2013
2012
2011
2010
2009
2008
2007
2006
2005
2004
2003
2002
2001
2000
1999
1998
1997
1995 - 1996
Volume 31, Number 6, June 2013
LC*GC June 2013 Table of Contents
COLUMNS
- COLUMN WATCH
The Inert Flow Path Story for GC and GC–MS: Eliminating the Weakest Links
Mitch Hastings and Ronald E. Majors
For the next improvements, we must look beyond the column.
- LC TROUBLESHOOTING
Gradient Elution, Part IV: Dwell-Volume Problems
John W. Dolan
How can the gradient dwell volume impact results?
- MS - THE PRACTICAL ART
Hydrogen–Deuterium Exchange Mass Spectrometry: An Emerging Biophysical Tool for Probing Protein Behavior and Higher-Order Structure
Joomi Ahn and St. John Skilton
HDX-MS as a means for performing routine biophysical analysis
- PERSPECTIVES IN MODERN HPLC
The Essence of Modern HPLC: Advantages, Limitations, Fundamentals, and Opportunities
Michael W. Dong
The fundamental concepts of HPLC are re-examined.
- THE ESSENTIALS
Troubleshooting GC Retention-Time, Efficiency, and Peak-Shape Problems
What problems in chromatogram may reveal, and what to do
ARTICLES
- A Tribute to Ron Majors
Laura Bush
For more than 40 years, he has been a master communicator. That’s just one reason he has so many fans, and friends, around the world.
- HPLC Analysis of Very Polar Compounds in Bioanalysis
Joe Pesek answers questions about IEX, HILIC, polar-embedded, and polar-endcapped columns, and aqueous normal phase chromatography.
Peer-Reviewed Article
- Morphological Comparison of Silica-Based Monolithic and Particulate Beds by Confocal Laser Scanning Microscopy
Stefan Bruns, Alexandra Höltzel, and Ulrich Tallarek
This analysis offers insight for optimizing the structure of monoliths.
DEPARTMENTS
- Peaks of Interest
- Product Resources
- Ad Index
Volume 31, Number 5, May 2013
LC*GC May 2013 Table of Contents
COLUMNS
- COLUMN WATCH
New Chromatography Columns and Accessories at Pittcon 2013, Part II
Ronald E. Majors
Gas chromatography columns, sample preparation products, and hardware, accessories, and small tabletop instruments mainly for sample preparation, are covered here.
- LC TROUBLESHOOTING
Gradient Elution, Part III: Surprises
John W. Dolan
Sometimes when changes are made to gradient conditions, the result isn’t what was expected.
- GC CONNECTIONS
New Gas Chromatography Products for 2013
John V. Hinshaw
Gas chromatography instruments and accessories that were newly on display at Pittcon 2013
- THE ESSENTIALS
HPLC Column Selection
Some “rules of thumb” can be applied to selecting stationary-phase type and column dimensions.
ARTICLES
- The 2013 LCGC Awards
Meg Evans
Profiles of Peter W. Carr, the Lifetime Achievement in Chromatography award winner, and Davy Guillarme, the Emerging Leader in Chromatography winner
- Evaluating the Temperature Shift in Analytical Temperature Rising Elution Fractionation
Adrian Boborodea and André Luciani
A new method is presented to evaluate the temperature shift observed in analytical temperature rising elution fractionation (ATREF).
DEPARTMENTS
- Peaks of Interest
- Product Resources
- Calendar
- Short Courses
- Chrom Corner
- Upcoming Webcasts
- Ad Index
Volume 31, Number 4, April 2013
LC*GC April 2013 Table of Contents
COLUMNS
- COLUMN WATCH
New Chromatography Columns and Accessories at Pittcon 2013, Part I
Ronald E. Majors
Part I of our yearly report, covering columns for all modes of HPLC, UHPLC, and liquid-phase separations.
- LC TROUBLESHOOTING
Gradient Elution, Part II: Equivalent Separations
John W. Dolan
Is it possible to get the same separation under isocratic and gradient conditions?
- MS - THE PRACTICAL ART
Nontargeted Metabolite Profiling by UHPLC–MS: A Case Study in Beer Reveals Effect of Temperature on Nonvolatile Small Molecules During Storage
Jessica Prenni, Adam Heuberger, and Dana Sedin
This analytical approach can be applied in many fields; the example illustrates its use in a food chemistry application.
- PERSPECTIVES IN MODERN HPLC
HPLC Systems and Modules Introduced at Pittcon 2013: A Brief Review
Michael W. Dong
A summary of new HPLC systems, modules, and software, focusing on innovations and unique features from a user’s perspective.
- THE ESSENTIALS
Troubleshooting GC Columns and Detectors
Help with common problems with preparation, installation, and care
ARTICLE
- Influence of Sample Solvent Composition for SFC Separations
Jacob N. Fairchild, Jason F. Hill, and Pamela C. Iraneta
A discussion of different sample solvent compositions to be injected for SFC as part of injection linearity experiments
DEPARTMENTS
- Peaks of Interest
- Product Showcase
- Calendar
- Ad Index
Volume 31, Number 3, March 2013
LC*GC March 2013 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
Trends in Sample Preparation
Ronald E. Majors
The results from a reader survey on sample preparation techniques
- LC TROUBLESHOOTING
Gradient Elution, Part I: Intuition
John W. Dolan
A how-to on understanding gradient elution
- GC CONNECTIONS
Measuring Gas Flow for Gas Chromatography
John V. Hinshaw
Comparison of gas flow measurement methods for GC laboratories
- THE ESSENTIALS
Column Selection for Reversed-Phase HPLC
How to choose the right stationary phase for your separation
ARTICLES
- Simple and Fast Quantification of Capsaicinoids in Hot Sauces Using Monolithic Silica Capillaries
and LC-MS
Simon Forster and Stephan Altmaier
Fast, easy, and resproducible determination of capsaicinoid content
- Determination of Sulfur-Based Odorants in Commercially Available Natural Gas with Flow-Modulated Comprehensive Two-Dimensional Gas Chromatography
T. Hayward, R. Gras, and J. Luong
A method able to quantitate odorants at levels as low as 100 ppb (v/v)
- Detection and Identification of Natural Antioxidants in Edible Oils Using LC Fractionation with Off-Line Effect-Based Detection
Ruud Poort, Herrald Steenbergen, and Hans-Gerd Janssen
Multiple fractions can be tested in parallel with this fast method.
- An Improved LC-MS-MS Method for the Determination of Clenbuterol in Human Urine
Jianghai Lu, Genye He, Xiaobing Wang, Youxuan Xu, Sheng Yang, Ying Dong, Yun Wu, Zhiyong Yang, Moutian Wu, and Gangfeng Ouyang
A sensitive method that reduces the number of false-positive results
- Identification of Organic Additives in Nitrile Rubber Materials by Pyrolysis-GC-MS
Peter Kusch
Small solid rubber samples can be analyzed without sample preparation.
DEPARTMENTS
- Peaks of Interest
- Product Resources
- Calendar
- Ad Index
Volume 31, Number 2, February 2013
LC*GC February 2013 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
Ionic Liquids and Their Applications in Sample Preparation
Tien D. Ho, Honglian Yu, William T.S. Cole, and Jared L. Anderson
Ionic liquids have tunable selectivity for specific classes of analytes, giving them distinct advantages over organic solvents.
- LC TROUBLESHOOTING
The Importance of the Sample Matrix
John W. Dolan
How to account for the sample matrix when developing a method
- MS - THE PRACTICAL ART
Identifying "Known Unknowns" in Commercial Products by Mass Spectrometry
James L. Little, Curtis D. Cleven, and Adam S. Howard
A systematic approach for the identification of nontargeted species, searching both mass spectral and "spectra-less" databases
- HISTORY OF CHROMATOGRAPHY
Man of the Masses
Kate Yu
An interview with Fred McLafferty, professor emeritus at Cornell University, about his pioneering career in mass spectrometry
- THE ESSENTIALS
Troubleshooting GC Gas Supply, Sample Preparation, and Inlets
The first three important stages in any GC analysis are explained.
ARTICLES
- Solvent Selection in Countercurrent Chromatography Using Small-Volume Hydrostatic Columns
Karine Faure, Nazim Mekaoui, Jeremy Meucci, and Alain Berthod
How to use small columns to test potential biphasic liquid systems for use in large-scale countercurrent chromatography separations
- LC-MS-Compatible Separation of Polar Compounds Using Silica Hydride Columns
Joshua E. Young, Hong Nhung Nguyen, Maria T. Matyska, Joseph J. Pesek
A guide to using HPLC in an iterative form to clarify decisionmaking during method development
DEPARTMENTS
- Peaks of Interest
- Product Showcase
- Calendar
- Short Courses
- Webcasts
- Ad Index
Volume 31, Number 1, January 2013
LC*GC January 2013 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
Approaches for Extracting and Determining Additives, Contaminants, and Low-Molecular-Weight By-Products in Synthetic Polymers
Howard G. Barth and Ronald E. Majors
Liquid-solid extraction is the most popular method, but sometimes modern approaches such as PLE/ASE and MAE are possible.
- LC TROUBLESHOOTING
Making the Most of a Gradient Scouting Run
John W. Dolan
How to choose conditions and what to do with the results
- GC CONNECTIONS
Gases Q & A, Part II
John V. Hinshaw
More questions about gases and their delivery to a GC instrument
- BIOTECHNOLOGY TODAY
Analytical Tools for Enabling Process Analytical Technology: HPLC and Quantitative Polymerase Chain Reaction
Rakesh Mendhe, Rahul Fadnis, Anurag S. Rathore, and Ira S. Krull
How to use these tools to develop an efficient and robust process
- THE ESSENTIALS
Solid-Phase Extraction
An explanation of each stage in a typical SPE protocol
ARTICLES
- Inaccuracies in the Quantification of Peptides - A Case Study Using β-Endorphin Assay
H.M.D.R. Herath, R.-R. Kim, P.J. Cabot, P.N. Shaw, and A.K. Hewavitharana
Likely sources of error are filtration and vial materials.
- Highlights of SFC 2012
Larry Miller and Larry Taylor
Food analysis, column selection, HILIC-SFC, and more
DEPARTMENTS
- Peaks of Interest
- Product Resources
- Calendar
- Ad Index