LC*GC

July 2009
June 2009
May 2009
April 2009
March 2009
February 2009
January 2009
2008
2007
2006
2005
2004
2003
2002
2001
2000
1999
1998
1997
1995 - 1996

COLUMNS

• SAMPLE PREP PERSPECTIVES
  Salting-out Liquid-Liquid Extraction (SALLE)
  Ronald E. Majors
  Ron Majors discusses the technique of Salting-out Liquid-Liquid Extraction (SALLE) and provides some examples of its successful application.
  
• LC TROUBLESHOOTING
  Calibration Curves, Part V: Curve Weighting
  John W. Dolan
  This is the fifth and final installment in a series of “LC Troubleshooting” columns about various aspects of calibration of liquid chromatographic (LC) methods.
  
• GC CONNECTIONS
  Pinning Down Tailing Peaks, Part II: Quantitative Measurement
  John V. Hinshaw
  Continuing a series on peak tailing in chromatography, John Hinshaw discusses the effects that peak tailing has on quantitative measurement of peak height and area.
  
• VALIDATION VIEWPOINT
  Analytical Method Validation for Biotechnology Proteins, Peptides, and Antibodies
  Michael Swartz and Ira Krull
  The authors explore how true (complete) method validation of biopharmaceuticals must be forthcoming.
  
• DIRECTIONS IN DISCOVERY
  Standardizing Proteomics
  Tim Wehr
  Following the sequencing of the human genome, the biological science community has moved to tackle the human proteome.
  

ARTICLE

• A Novel Sample Preparation Approach to Increase the Throughput of Pesticide Analysis by LC–MS-MS
  Joseph McClory, Robert Henze, Keith Tucker, and Robert Thomas
  The authors evaluate a new approach to sample preparation for the analysis of pesticides in raw agricultural commodities.
  

DEPARTMENTS

• From the Editor
• Peaks of Interest
• Products
• Literature
• Classified Directory
• Ad Index

COLUMNS

• COLUMN WATCH
  The Continuing Acetonitrile Shortage: How to Combat it or Live with It
  Ronald E. Majors
  Ron Majors provides an unbiased look at this present crisis and gives alternative solutions with the intent to reduce (not necessarily eliminate) the use of copious amounts of acetonitrile in HPLC and sample preparation.
  
• LC TROUBLESHOOTING
  Calibration Curves, Part IV: Choosing the Appropriate Model
  John W. Dolan
  Part IV of this series focuses on three different calibration models: external standardization, internal standardization, and the method of standard additions.
  
• MS — THE PRACTICAL ART
  Open Access, Automation, and the Promise of Simple Complexity
  Michael P. Balogh
  Columnist Michael Balogh looks at the questions: Where are we now? What do the early MS innovators see as their legacy? And what can we expect according to those who now have 15 or 20 years of experience behind them?
  

ARTICLES

• ASMS Review
  Megan Evans
  Assistant Editor Megan Evans takes a look back at the annual ASMS Conference that was recently held in Philadelphia.
  
• Separation of Ionic Analytes via Supercritical Fluid Chromatography: Achieving the Impossible
  Larry T. Taylor
  The author looks at both the history and the recent developments in supercritical fluid chromatography (SFC), which has been practiced for nearly a half century.
  

DEPARTMENTS

• Peaks of Interest
• Products
• Literature
• Classified Directory
• Ad Index

COLUMNS

• SAMPLE PREP PERSPECTIVES
  Stir Bar Sorptive Extraction (SBSE) and Headspace Sorptive Extraction (HSSE): An Overview
  Carlo Bicchi, Erica Liberto, Chiara Cordero, Barbara Sgorbini, and Patrizia Rubiolo
  The guest columnists review SBSE and HSSE and examine their advantages and limitations, along with providing approaches to overcoming the latter.
  
• LC TROUBLESHOOTING
  Calibration Curves III: A Different View
  John W. Dolan
  This is the third installment in a series of LC Troubleshooting columns that focus on calibration curves used for liquid chromatographic (LC) methods. Here, John Dolan considers some alternate ways to look at the data to determine if they appear to be normal or are trying to tell us that something is amiss.
  
• GC CONNECTIONS
  Pittcon 2009 GC Systems and Accessories Review
  John V. Hinshaw
  John Hinshaw presents his review of all that was new and innovative at Pittcon 2009 in the field of gas chromatography.
  

ARTICLES

• Evaporation-Free Extraction and Application in High-Throughput Bioanalysis by LC–MS-MS
  Aimin Tan, Saleh Hussain, and François Vallée
  Presented here are four types of evaporation-free extraction that are widely applicable.
  
• Effects of Sample-Dissolving Solvents and Sample Ionic Strength in Routine CE Operation
  Kevin Altria
  The ionic strength of a sample and the solvent used to dissolve it play a pivotal role in routine capillary electrophoresis (CE).
  

DEPARTMENTS

• From the Editor
• Peaks of Interest
• Products
• Literature
• Classified Directory
• Ad Index

COLUMNS

• COLUMN WATCH
  New Chromatography Columns and Accessories at Pittcon 2009: Part II
  Ronald E. Majors
  Ron Majors brings readers the second part of his yearly review of all that was new and innovative at the annual Pittsburgh Conference.
  
• LC TROUBLESHOOTING
  Calibration Curves, Part II: What are the Limits?
  John W. Dolan
  In part two of this series, John Dolan looks at the signal-to-noise ratio and its relationship to uncertainty in a measurement.
  
• MS — THE PRACTICAL ART
  Alternative Futures: Biofuels and Mass Spectrometry
  Michael P. Balogh
  The use of mass spectrometry to study biofuels is examined and the advantages and disadvantages are explored.
  
• VALIDATION VIEWPOINT A Quality-by-Design Methodology for Rapid LC Method
  Development, Part III
  Joseph Turpin, Patrick H. Lukulay, and Richard Verseput
  The guest columnists continue their examination of how statistically rigorous QbD principles can be put into practice.
  

ARTICLE

• Approaches to Singleton Achiral Purification of Difficult Samples for Discovery Research Support in the Pharmaceutical Industry
  Tony Q. Yan, Jim Bradow, Shang-Poa Chang, Rob Depianta, and Laurence Philippe
  Several approaches for purifying difficult samples more efficiently for discovery research support are mentioned in this paper. These approaches use mass triggered high performance liquid chromatography (HPLC) on various specialty columns.
  

DEPARTMENTS

• Peaks of Interest
• Products
• Literature
• Calendar
• Short Courses
• Classified Directory
• Ad Index

COLUMNS

• COLUMN WATCH
  New Chromatography Columns and Accessories at Pittcon 2009: Part I
  Ronald E. Majors
  Ron Majors brings readers his yearly review of all that was new and innovative at the annual Pittsburgh Conference.
  
• LC TROUBLESHOOTING
  Calibration Curves, Part I: To b or Not to b?
  John W. Dolan
  This month’s “LC Troubleshooting” looks at some different calibration models, how to decide if a calibration curve goes through zero, and some problems that can occur if the wrong choices are made.
  
• GC CONNECTIONS
  Pinning Down Tailing Peaks
  John V. Hinshaw
  The causes and cures for peak tailing are discussed, along with the various ways that chromatographers measure tailing peaks.
  
• DIRECTIONS IN DISCOVERY
  Electrostatic Repulsion Hydrophilic Interaction Chromatography — A New Tool for Enrichment of Phospho- and Glycopeptides
  Tim Wehr
  ERLIC is a new technique that shows promise as an alternative or complementary technique for both enrichment and separation of these posttranslationally-modified peptides.
  

ARTICLES

• The 2009 LCGC Awards
  David Walsh
  LCGC hands out a pair of awards at Pittcon 2009 in Chicago.
  
• Separation, Identification, and Quantitation of Water-Soluble Vitamins by HPLC
  Karyn M. Usher, Anna Glinko, Michael J. Bozym, and Michelle L. Owens
  The authors set out to perform a separation of seven water-soluble vitamins without the use of ion-pair reagents.
  

DEPARTMENTS

• Peaks of Interest
• Products
• Literature
• Classified Directory
• Ad Index

COLUMNS

• SAMPLE PREP PERSPECTIVES
  Extraction and Preconcentration of Residues and Contaminants in Food Samples Using Membrane Techniques
  Titus A.M. Msagati, Luke Chimuka, Ewa Cukrowska, and Hlanganani Tutu
  Guest authors from South Africa review the application of membranes in the extraction, preconcentration, and separation of various contaminants in foods.
  
• LC TROUBLESHOOTING
  Testing Method Performance
  John W. Dolan
  This month’s “LC Troubleshooting” discussion will center on the recommendations of the CDER document, especially in terms of what it means from a practical method performance standpoint.
  
• MS — THE PRACTICAL ART
  A Mass Spectrometry Primer, Part IV
  Michael P. Balogh
  This column completes the multipart MS primer with a list of terms derived from common usage throughout various industries.
  
• HISTORY OF CHROMATOGRAPHY
  The Early Days of HPLC at DuPont
  Richard A. Henry
  Guest columnist Dick Henry traces the history of HPLC back more than 40 years, starting with his days at DuPont.
  

ARTICLES

• Facilitating Discovery of Prion Disease Biomarkers by Quantitative Glycoproteomics
  Xin Wei, Allen Herbst, Joshua Schmidt, Judd Aiken, and Lingjun Li
  The authors examine MS-based methods to search for protein biomarkers of prion diseases from plasma samples.
  
• ICP–MS Detection for HPLC Analyses of Pharmaceutical Products
  Tim Shelbourn, Leah Williamson, Todd Gillespie, and Robert Montgomery
  The authors investigate applications for ICP-MS detection with reversed-phase HPLC.
  

DEPARTMENTS

• From the Editor
• Peaks of Interest
• Products
• Literature
• Calendar
• Short Courses
• Classified Directory
• Ad Index

COLUMNS

• COLUMN WATCH
  Improving Protein Separations with Mixed-Mode Chromatography
  Andrew Lees and Marty Burkhardt
  Guest authors show how mixed modes can be used successfully in the optimization of protein purification, and discuss how various experimental parameters such as pH, buffer choice, and buffer strength can be used to regulate the binding of proteins to mixed-mode sorbents.
  
• LC TROUBLESHOOTING
  Problems from East and West
  John W. Dolan
  At the beginning of the year, it is good to look back in time — are there things that we can learn from the past? After a series of troubleshooting and method development seminars in Istanbul, Turkey and Amman, Jordan, John Dolan looks back.
  
• GC CONNECTIONS
  Optimizing Detector Setup and Operating Conditions
  John V. Hinshaw
  This month, John Hinshaw examines the environment, setup, and operating conditions necessary to ensure high gas chromatography detector performance and reliability.
  
• VALIDATION VIEWPOINT
  A Quality-by-Design Methodology for Rapid LC Method Development, Part II
  Joseph Turpin, Patrick H. Lukulay, and Richard Verseput
  This second part of the series describes the data loss inherent in most early method development experiments due to coelution, peak exchange, and the general difficulty of accurately identifying peaks across the experiment trial chromatograms.
  

ARTICLE

• Asymmetric Flow Field Flow Fractionation: A Powerful Method for Polymer Characterization
  Stepan Podzimek, Petr Lebeda, and Christoph Johann
  Asymmetric flow field flow fractionation is a type of field flow fractionation (FFF) separation technique. FFF has been co-existing with size exclusion chromatography (SEC) for several decades.
  

DEPARTMENTS

• Peaks of Interest
• Products
• Literature
• Calendar
• Short Courses
• Classified Directory
• Ad Index