LC*GC
December 2004
November 2004
October 2004
September 2004
August 2004
July 2004
June 2004
May 2004
April 2004
March 2004
February 2004
January 2004
2003
2002
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1995 - 1996
Volume 22, Number 12, December 2004
LC*GC December 2004 Table of Contents
COLUMNS
- COLUMN WATCH
Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part I: Changing the Column
Lloyd R. Snyder and John W. Dolan
The authors look at two types of method modification from a scientific and regulatory standpoint: a change of column and a change in the operating system. Both are difficult to avoid if a method is used for several years.
- LC TROUBLESHOOTING
Retention Time Changes
John W. Dolan
While some variation in retention is normal, larger changes from run to run are generally indicative of a problem. Here, the author discusses how the retention time of a peak in an LC separation can be a useful diagnostic tool to identify problems with a separation.
- GC CONNECTIONS
Flow, Pressure, and Temperature Calibration: Part I
John Hinshaw
In this first of a two-part installment, John Hinshaw discusses the relative effects that inlet pressures and oven temperatures have on capillary gas chromatography.
EDITORIAL INDEX
- 2004 Index of Authors and Subjects
DEPARTMENTS
- Peaks of Interest
- Products
- Classified Directory
- Ad Index
Volume 22, Number 11, November 2004
LC*GC November 2004 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
New Developments in Microplates for Biological Assays and Automated Sample Preparation
Ronald E. Majors
The author focuses on some key considerations in the manufacture and use of microplates, particularly the 96-well plate.
- LC TROUBLESHOOTING
My Favorite Shortcuts
John W. Dolan
A detailed look at some helpful shortcuts for estimating chromatographic parameters.
- MS -- THE PRACTICAL ART
Emerging Technologies in the MS Arsenal
Michael P. Balogh
Several recently developed technologies are described, along with the ways they can improve the utility and performance of mass spectrometers.
ARTICLES
- Determining Sulfamethazine in Pork Using HPLC with a 100% Water Mobile Phase
Naoto Furusawa and Kunihiro Kishida
The authors describe a method for determining sulfamethazine residue in pork using 100% water in both sample preparation and HPLC separation.
- Single-Compound Isotopic Analysis of Organic Materials in Archaeology
Jennifer A. Tripp and Robert E.M. Hedges
The authors review chromatographic separations of archaeological bio-molecules for their analysis using accelerator mass spectrometry or isotope ratio mass spectrometry.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Web Showcase
- Short Courses
- Calendar
- Classified Directory
- Ad Index
Volume 22, Number 10, October 2004
LC*GC October 2004 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
Practical Tips on Preparing Plasma Samples for Drug Analysis Using SPME
Maria Eugênia C. Queiroz and Fernando M. Lanças
- LC TROUBLESHOOTING
Gradient Performance Checks
Jonathan J. Gilroy and John W. Dolan
- GC CONNECTIONS
Finding a Needle in a Haystack
John Hinshaw
- DIRECTIONS IN DISCOVERY
Coupling Capillary Isoelectric Focusing with Mass Spectrometry
Tim Wehr
ARTICLES
- Validation and Implementation of In-Process Control HPLC Assays for Active Pharmaceutical Ingredients
P.W. Wrezel, I. Chion, and M.S. Hussain
The authors describe a sensible approach for validation and implementation of HPLC IPC assays.
- Hydrophilic Interaction Chromatography Using Silica Columns for the Retention of Polar Analytes and Enhanced ESI-MS Sensitivity
Eric S. Grumbach, Diane M. Wagrowski-Diehl, Jeffrey R. Mazzeo, Bonnie Alden, and Pamela C. Iraneta
The use of a silica column for HILIC is examined, including mechanistic studies of analyte retention on silica in aqueous–organic mobile phases.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Short Courses
- Calendar
- Classified Directory
- Ad Index
Volume 22, Number 9, September 2004
LC*GC September 2004 Table of Contents
COLUMNS
- COLUMN WATCH
HPLC 2004: A Review of New Columns, Equipment, and Analytical Developments
Ronald E. Majors
- LC TROUBLESHOOTING
Do Your LC Habits Make Sense?
John W. Dolan
- MS THE PRACTICAL ART
Taming the Regulatory Beast: Regulation vs. Functionalism
Michael P. Balogh and Virgina L. Corbin
- MILESTONES IN CHROMATOGRAPHY
The Evolution of Capillary Gel Electrophoresis
Andras Guttman and Leslie S. Ettre
- VALIDATION VIEWPOINT
Easing the Burden of GMP Compliance
Michael Swartz and Ira Krull
ARTICLES
- Polybrominated Diphenyl Ether Analysis in Fish Tissue and Other Matrices Using GC-ECD
R.H. Rieck
The author describes the development of a method for the extraction, clean-up, and successful analysis of polybrominated diphenyl ethers (PBDEs) in fish tissue, soil, and water.
- Recent Advances in Bioanalytical Mass Spectrometry and Their Application to Marine Toxins
Dietrich A. Volmer and Lekha Sleno
An overview of some recently developed mass spectrometry techniques and their application to marine biotoxins.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Short Courses
- Calendar
- Classified Directory
- Ad Index
Volume 22, Number 8, August 2004
LC*GC August 2004 Table of Contents
LCGC Buyers' Guide
Product Locator
Products and Services
- Liquid Chromatography
- Gas Chromatography
- Supercritical Fluid Chromatography
- Thin-Layer Chromatography
- Electrophoresis
- Reagents, Solvents, Standards
- Data Handling, Calibration
- Chromatography Services
- Sample Preparation and Handling
- General Scientific Equipment and Accessories
Manufacturers Directory
Cyber Directory
Classified Directory
Advertisers Index
Volume 22, Number 7, July 2004
LC*GC July 2004 Table of Contents
COLUMNS
- COLUMN WATCH
Developments in Preparative-Scale Chromatography Columns and Accessories
Ronald E. Majors
A look at the latest developments in preparative-scale columns, bulk packing materials, and column hardware designs.
- LC TROUBLESHOOTING
Divide and Conquer
John W. Dolan
The author focuses on a simple but powerful tool for isolating problems in the laboratory.
- GC CONNECTIONS
The Retention Gap Effect
John V. Hinshaw
The effects of adding a precolumn –– or retention gap –– to a capillary column are explored.
ARTICLES
- Stability Evaluations of an Octadecyl Bonded Phase Based upon Titanium-Grafted Silica
César Ricardo Silva, Claudio Airoldi, Kenneth E. Collins, and Carol H. Collins
A C18 stationary phase based upon a titanized silica support was prepared through reaction with octadecyltrimethoxysilane.
- Molecular Movement in an HPLC Column: A Stochastic Analysis
Attila Felinger
The authors use the stochastic model to estimate the fundamental characteristics of the separation process.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Short Courses
- Calendar
- Classified Directory
- Ad Index
Volume 22, Number 6, June 2004
LC*GC June 2004 Table of Contents
COLUMNS
- SAMPLE PREP PERPSPECTIVES
Headspace Liquid Microextraction
Derrick C. Wood, James M. Miller, and Ingo Christ
- LC TROUBLESHOOTING
But I Can’t Change the Method
John W. Dolan
- MS –– THE PRACTICAL ART
Finding the Right Forum to Learn about LC–MS
Michael P. Balogh
- VALIDATION VIEWPOINT
Setting Specifications
Michael Swartz and Ira Krull
ARTICLES
- Solubility of Buffers in Aqueous–Organic Eluents for Reversed-Phase Liquid Chromatography
Adam P. Schellinger and Peter W. Carr
The authors study the solubility of five buffers commonly used in reversed-phase liquid chromatography.
- The Analysis of Fluorescent Whitening Agents Using Reversed-Phase HPLC and Mass Spectrometry
M. de los Santos, C. Nerín, C. Domeño, and R. Batlle
The separation of individual congeners of commonly used fluorescent whitening agents is described.
- A Study of Regulators for Delivering Gases Containing Low Concentrations of Hydrogen Sulfide
Bruce Talbert, Robert Benesch, Malik Haouchine, and Tracey Jacksier
The authors discuss how the materials, construction, and design of gas regulators can affect the delivery of low-level reactive gas mixtures.
DEPARTMENTS
- Peaks of Interest
- In Memory
- Products
- Literature
- Short Courses
- Calendar
- Classified Directory
- Ad Index
Volume 22, Number 5, May 2004
LC*GC May 2004 Table of Contents
COLUMNS
- COLUMN WATCH
The Role of the Column in Preparative HPLC
Ronald E. Majors
A look at some approaches to scaling up from an analytical column to a preparative column.
- LC TROUBLESHOOTING
System Suitability
John W. Dolan
A discussion of system-suitability testing guidelines and how to apply them in the lab.
- GC CONNECTIONS
New Products and Trends in Gas Chromatography at Pittcon 2004
John V. Hinshaw
A round-up of GC product introductions from Pittcon 2004.
- MILESTONES IN CHROMATOGRAPHY
More Speed, Better Precision, Higher Sensitivity: Why Buy a New Gas Chromatograph?
Leslie S. Ettre and Werner Engewald
Developments in gas chromatography instrumentation, columns, and performance during the past 10 years.
ARTICLE
- Rapid Determination of Terpene Lactones in Ginkgo Biloba Commercial Products by HPLC with Evaporative Light-Scattering Detection
Tim Herring
This article presents a rapid, sensitive, and reproducible HPLC gradient method for the measurement of ginkgolides A, B, C, and J, along with bilobalide, in Ginkgo biloba commercial products.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Calendar
- Web Spotlight
- Short Courses
- Classified Directory
- Ad Index
Volume 22, Number 4, April 2004
LC*GC April 2004 Table of Contents
COLUMNS
- COLUMN WATCH
New Chromatography Columns and Accessories at the 2004 Pittcon Conference, Part II
Ronald E. Majors
The second of a two-part series examining new column introductions at Pittcon 2004.
- LC TROUBLESHOOTING
Parallel Chromatography — Double Your Money
Michael D. Nelson and John W. Dolan
A look at ways to get more out of your detector.
- MS –– THE PRACTICAL ART
Getting the Most for the Effort . . . Quickly
Diane M. Diehl and Michael P. Balogh
Increase efficiency, not frustration, in small-molecule practices.
- DIRECTIONS IN DISCOVERY
HPLC Standard Controls as a Requirement in Proteomics
Karen M. Gooding, Barry Hodge, and Randall K. Julian, Jr.
How the use of peptide standards can indicate control as well as provide guidelines for method development.
ARTICLES
- Simultaneous Determination of Ascorbic Acid and Free Malondialdehyde in Human Serum by HPLC–UV
Mustafa Karatepe
A simple, rapid, and sensitive method is presented for clinical measurements of ascorbic acid and malondialdehyde in human serum.
- An Introduction to OPLC Operation and Applications
Nathan Bryson and Domitille Papillard
This article describes the optimum performance laminar chromatography technique and gives a brief account of recent applications in which it has been used.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Calendar
- Short Courses
- Classified Directory
- Ad Index
Volume 22, Number 3, March 2004
LC*GC March 2004 Table of Contents
COLUMNS
- COLUMN WATCH
New Chromatography Columns and Accessories at the 2004 Pittcon Conference, Part I
Ronald E. Majors
This first of a two-part series examining new column introductions at Pittcon 2004 looks at high performance liquid chromatography (HPLC) columns and packings for reversed-, normal-, and bonded-phase; ion-exchange; ion; and size-exclusion chromatography; and specialty HPLC columns.
- LC TROUBLESHOOTING
Success with Evaporative Light-Scattering Detection, Part II: Tips and Techniques
Craig S. Young and John W. Dolan
In this continuation of a previous column on evaporative light-scattering detection, common questions about the technique are answered and further detector applications are discussed.
- GC CONNECTIONS
Anatomy of a Peak
John V. Hinshaw
This discussion of chromatographic peak anatomy pays particular attention to features that help determine the suitability of individual chromatographs for a specific analysis task.
- MILESTONES IN CHROMATOGRAPHY
Forerunners of Chromatography: Runge’s Self-Grown Pictures
Heinz H. Bussemas and Leslie S. Ettre
A look at the activities of F.F. Runge, a 19th century scientist whose investigations into colors and dyes could be considered a precursor of chromatography.
ARTICLE
- Applying Supersaturated Experimental Designs to the Study of Composite Sampling for Monitoring Pesticide Residues in Water
Rosario Rodil, Elena Martínez, Antonia Maria Carro, Rosa Antonia Lorenzo, and Rafael Cela
The authors propose the use of supersaturated experimental designs for composite sampling and demonstrate their efficiency and economy in the environmental screening of pesticide residues.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Classified Directory
- Ad Index
Volume 22, Number 2, February 2004
LC*GC February 2004 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
Speciation Studies of Metal Ions in Environmental and Biological Media Using Supported Liquid Membrane Extraction
Luke Chimuka
- LC TROUBLESHOOTING
Selecting the Best Curve Fit
Melissa M. Kiser and John W. Dolan
- MS – THE PRACTICAL ART
Debating Resolution and Mass Accuracy
Michael P. Balogh
- VALIDATION VIEWPOINT
Investigating Out-of-Specification Results
Michael Swartz and Ira Krull
ARTICLES
- Determination of Common Inorganic Anions in Environmental Waters Using a Hydroxide-Selective Column
Brian M. De Borba, Douglas W. Later, Jeff S. Rohrer, and Chris A. Pohl
The authors evaluate the performance of a high-capacity anion-exchange column for fulfilling the requirements of EPA Method 300.0 Part A and compare the results with data generated with an anion exchange column.
- Estimation of the Main Sources of Uncertainty in Chromatographic Analysis: Determination of Biogenic Amines
R. Romero, M. G. Bagur, D. Gázquez, M. Sánchez-Viñas, L. Cuadros-Rodríguez, and M. Ortega
This article establishes the estimation of the uncertainty associated with the chromatographic determination of biogenic amines.
- Developments in 2-D Gas Chromatography with Heartcutting
Kevin MacNamara, Riccardo Leardi, and Andreas Hoffmann
The authors present 2-D gas chromatography (GC) with heartcutting as a powerful tool for routine analysis of complex samples.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Pittcon Product Showcase
- Classified Directory
- Calendar
- Ad Index
Volume 22, Number 1, January 2004
LC*GC January 2004 Table of Contents
- Sample Prep Perspectives
In-Tube Solid-Phase Microextraction and On-Line Coupling with High-Resolution GC
Hanwen Wang, Wenmin Liu, and Yafeng Guan
- LC Troubleshooting
Broad Peaks
John W. Dolan
- GC Connections
Comprehensive Two-Dimensional Gas Chromatography
John V. Hinshaw
- Milestones in Chromatography
The Evolution of the Application of Gas Chromatography
Leslie S. Ettre
- An Improved Analytical Temperature-Rising Elution Fractionation System for Automated Analysis of Polyethylenes
Adrian G. Boborodea, Daniel Daoust, Alain M. Jonas, and Christian Bailly
- Analysis of Combinatorial Natural Products by HPLC and CE
András Guttman, Julia Khandurina, Paul Budworth, Wenying Xu