**LC*GC**

December 2005
November 2005
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June 2005
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Volume 23, Number 12, December 2005

**LC*GC December 2005 Table of Contents**

COLUMNS

COLUMN WATCH
Fast and Ultrafast HPLC on Sub-2-m Porous Particles - Where Do We Go From Here?
Ronald E. Majors
In this month's "Column Watch," Ron Majors traces the development of particle technology from the beginning of HPLC to the present.
LC TROUBLESHOOTING
The Role of Signal-to-Noise Ratio in Precision and Accuracy
John W. Dolan
John Dolan examines the measurement of S/N, some techniques for determining the detection limits for a method, and some tricks to improve S/N.
GC CONNECTIONS
The Flame Ionization Detector
John V. Hinshaw
In this installment of "GC Connections," the author takes a look at the workings of FID and discusses problem determination and resolution.
MILESTONES IN CHROMATOGRAPHY
Early Petroleum Chromatographers
Leslie S. Ettre
This column deals with a few chemists active during the early growth of the petroleum industry who carried out chromatography-like investigations even before Tswett.

EDITORIAL INDEX

2005 Index of Authors and Subjects

DEPARTMENTS

Peaks of Interest
From the Editor
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Volume 23, Number 11, November 2005

**LC*GC November 2005 Table of Contents**

COLUMNS

COLUMN WATCH
Successful Gas Chromatography Using Fused-Silica Capillary Columns
Rick Parmely
Guest columnist Rick Parmely takes a look at some capillary column basics of a well-established separation technique. He discusses peak tailing, column overload, ghost peaks, and column bleed and speculates on "when to give up."
LC TROUBLESHOOTING
Extending Column Life
John W. Dolan
No column lasts forever, but in this month's installment of "LC Troubleshooting," John Dolan offers some tricks to help extend a column's life.
DIRECTIONS IN DISCOVERY
Capillary Electrophoresis in the Biopharmaceutical Industry: Part II
Tim Wehr
In this, the second of a two-part series, Tim Wehr explores the remaining techniques that are replacing gel electrophoresis. The challenges facing this technicque also are discussed, including management acceptance, technical hurdles, and the need for training.

ARTICLE

Determination of Stilbenes in Blueberries
Agnes M. Rimando and Robert Cody
There is growing evidence that blueberries have powerful disease-fighting properties. Here, the authors explore the reaearch being done in this area, describing the use a magnetic sector GC-MS SIM method to determine the presence of phenolic antioxidants in blueberries.

DEPARTMENTS

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Volume 23, Number 10, October 2005

**LC*GC October 2005 Table of Contents**

COLUMNS

COLUMN WATCH
Multidimensional and Comprehensive Liquid Chromatography
Ronald E. Majors
Ron Majors discusses advances in multidimensional chromatography and comprehensive multidimensional chromatography. In particular, the bright future of LC LC, a technique currently in its infancy, is discussed.
LC TROUBLESHOOTING
LC Troubleshooting: The Basics
John W. Dolan
This month's "LC Troubleshooting" is aimed at those who are just starting out in the liquid chromatography business, as John Dolan presents some helpful tips.
MS - THE PRACTICAL ART
Profiles in Practice: Advances in Science and Geopolitical Issues
Michael P. Balogh
Few areas of LC-MS practice have been so affected by geopolitics as that of food safety analysis, a topic as important as it is complex. Here, Michael Balogh examines this topic.
VALIDATION VIEWPOINT
Validation, Qualification, or Verification?
Michael Swartz and Ira Krull
The authors take a look at three recently published documents in the validation literature from groups working to (hopefully!) clear up some of the potential for confusion.

ARTICLE

A Laser Diode Double-Pumped Solid-State Laser-Induced Fluorescence Detector for Capillary Electrophoresis and Liquid Chromatography
Bingcheng Yang, Feng Tan, and Yafeng Guan
The authors describe a new laser-induced fluorescence detector for application to both both capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC).

DEPARTMENTS

From the Editor
Peaks of Interest
Products
Literature
Calendar
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Volume 23, Number 9, September 2005

**LC*GC September 2005 Table of Contents**

COLUMNS

COLUMN WATCH
Highlights of HPLC 2005
Ronald E. Majors
HPLC 2005 was held in Stockholm, Sweden, June 26-30. In this month's installment of "Column Watch," Ron Majors covers this year's hot topics including proteomics, column technology - especially monoliths - microfluidics, and multidimensional and high-throughput separations.
LC TROUBLESHOOTING
A Baker's Dozen
John W. Dolan
Some simple practices will help to make problems with gradient methods rare rather than frequent. This month's installment of "LC Troubleshooting" presents a baker's dozen of these tips.
GC CONNECTIONS
The Making of a Column
John V. Hinshaw
In this installment of "GC Connections," the author review some of the developments in fused-silica column technology and then takes a brief tour through the process of column manufacturing.

ARTICLE

Evaluating Analytical Instrumentation Using the Golf Score Card Tool
Timothy R. Baker, Steven H. Hoke, II, and Roy L. M. Dobson.
The use of a simple Excel-based "Golf Score Card" tool can facilitate assessments and instrument acquisition decisions by collating and weighting the relative importance of the many criteria that impact the final purchase decision. The authors describe this process.

DEPARTMENTS

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Volume 23, Number 8, August 2005

**LC*GC August 2005 Table of Contents**

COLUMNS

SAMPLE PREP PERSPECTIVES
Sample Preparation for Large-Scale Protein Purification
Ronald E. Majors
Columnist Ron Majors discusses methods that can be used to isolate large amounts of proteins from crude biological samples.
LC TROUBLESHOOTING
Broad Peaks
John W. Dolan
The author takes a look at the problem of unusually broad peaks and covers some techniques that will help determine the reasons behind them.
MS - THE PRACTICAL ART
Profiles in Practice Series: A Revolution in Clinical Chemistry
Michael P. Balogh
The applications of mass spectrometry to clinical chemistry are discussed with the help of this month's featured scientists.
MILESTONES IN CHROMATOGRAPHY
Jim Waters: The Development of GPC and the First HPLC Instruments
Leslie S. Ettre
Leslie Ettre looks at the life of industry giant Jim Waters and his contributions to the development of HPLC.

ARTICLE

Evaluation of Polar-Embedded and Polar-Endcapped C12-C18 Stationary Phase Vulnerabilities Toward Highly Nucleophilic Compounds: A Case Study
Larry J. Burge and Christina M. Sorgen
Here the authors discuss an ion-pair, gradient HPLC method, developed for cycloserine-related substances using a polar, amide-embedded C18 column. Other polar-embedded and polar-endcapped columns are evaluated in equivalence studies.

DEPARTMENTS

From the Editor and Publisher
Peaks of Interest
Letters to the Editor
Products
Literature
Calendar
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Classified Directory
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Volume 23, Number 7, July 2005

**LC*GC July 2005 Table of Contents**

COLUMNS

SAMPLE PREP PERSPECTIVES
Micropipette Tip-Based Sample Preparation for Bioanalysis
Ronald E. Majors and Ashok Shukla
The authors review the latest technologies in MPT sample preparation used in the study of genomics, proteomics, and metabolomics.
LC TROUBLESHOOTING
Reader Questions: Mobile Phase
John W. Dolan
Columnist John Dolan explores several issues related to mobile phase, using readers' questions as the basis for the discussion.
GC CONNECTIONS
How To Buy Gas Calibration Mixes
Steve Scheuring and Daniel Bartel
This month's guest authors update a previous installment of "GC Connections," discussing methods for selecting pure gases for analytical applications.
DIRECTIONS IN DISCOVERY
Capillary Electrophoresis in the Biopharmaceutical Industry: Part I
Tim Wehr
In this, the first of a two-part series, the author reports how capillary electrophoresis is coming to be the preferred method for product characterization and QC.

ARTICLE

Key Factors in Sample Diluent Selection for HPLC Assays of Active Pharmaceutical Ingredients
P.W. Wrezel, I. Chion, and R. Pakula
The authors explain why sample diluent is more than just a solubilizing agent and describe a systematic process for diluent selection when developing an HPLC assay of an active pharmaceutical ingredient.

DEPARTMENTS

Peaks of Interest
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Volume 23, Number 6, June 2005

**LC*GC June 2005 Table of Contents**

COLUMNS

COLUMN WATCH
Fluorinated HPLC Phases - Looking Beyond C18 for Reversed-Phase HPLC
Matthew Przybyciel
Guest columnist Matt Przybyciel reviews the structure, behavior, and applications of both alkyl- and phenyl-fluorinated phases in HPLC.
LC TROUBLESHOOTING
Peak Fronting, Column Life, and Column Conditioning
Ryan D. Morrison and John W. Dolan
The authors look at three column performance problems encountered frequently in today's laboratory.
MS - THE PRACTICAL ART
Profiles in Practice Series: The High-Speed State of Information and Data Management
Michael P. Balogh
Columnist Michael Balogh explores the topics of informatics and data management with this month's featured scientists.
VALIDATION VIEWPOINT
Developing and Validating Stability-Indicating Methods
Michael Swartz and Ira Krull
The authors outline the ways in which forced degradation is used to develop a stability-indicating method and examine some of the method development and validation parameters critical to its success.

ARTICLE

Enhanced Sample Throughput for Environmental Analysis
Joeri Vercammen, Kelly François, Michèle Wolf, An Vermeulen, and Katrien Welvaert
As environmental legislation becomes more stringent, the need to deliver quantitative results in shorter times and greater volumes is necessary for routine environmental analysis. Here, the authors explore several ways to accomplish this.

DEPARTMENTS

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Volume 23, Number 5, May 2005

**LC*GC May 2005 Table of Contents**

COLUMNS

COLUMN WATCH
Stationary Phases for Planar Separations -- Plates for Modern TLC
Eike Reich and Anne Schibli
The authors provide the latest information on new stationary phases for modern TLC and high performance TLC (HPTLC), along with helpful hints on how to get the most out of this flexible form of chromatography.
LC TROUBLESHOOTING
Overload or Minor Peak?
John W. Dolan
Some possible solutions to the ubiquitous peak-tailing problem are presented by the author.
GC CONNECTIONS
New GC Instruments and Accessories at the 56th Pittsburgh Conference
John V. Hinshaw
A review of the latest products and innovations in gas chromatography to come out of Pittcon 2005.
MILESTONES IN CHROMATOGRAPHY
Csaba Horváth and the Development of the First Modern High Performance Liquid Chromatograph
Leslie S. Ettre
Columnist Leslie Ettre looks back at the life and contributions of Csaba Horváth, one of the giants in the field of chromatography.

ARTICLE

Important Guidelines for Optimizing Speed and Sensitivity in Small-Molecule LC-UV and LC-MS
Richard A. Henry and David S. Bell
As LC-UV and LC-MS have become preferred instrumental methods for fast, sensitive analysis, many scientists are searching for ways to optimize performance. Here, the authors discuss the ways in which HPLC with sensitive detectors is solving new problems in high-speed analysis.

DEPARTMENTS

Peaks of Interest
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Literature
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Volume 23, Number 4, April 2005

**LC*GC April 2005 Table of Contents**

COLUMNS

COLUMN WATCH
New Chromatography Columns and Accessories at the 2005 Pittcon Conference: Part II
Ronald E. Majors
LC TROUBLESHOOTING
The Bermuda Triangle
John W. Dolan
MS - THE PRACTICAL ART
Profiles in Practice Series: Stewards of Drug Discovery - Developing and Maintaining the Future Drug Candidates
Michael. P. Balogh

ARTICLES

A High-Volume, High-Throughput LC-MS Therapeutic Drug Monitoring System
Kolbjorn Zahlsen, Trond Aamo, and Jerry Zweigenbaum
LC-MS monitoring of the drug clozapine is detailed along with a description of the overall system architecture, workflow, and maintenance routines that support a large-scale drug monitoring program.
Control of Matrix Effects in Bioanalytical MS-MS Using On-line Multidimensional Solid-Phase Extraction
Katrin Georgi and Karl-Siegfried Boos
The authors discuss a solid-phase extraction (SPE) clean-up procedure for drugs present in biofluids.
Analysis of Fusarium Toxins Using LC-MS-MS: Application to Various Food and Feed Matrices
Scarlett Biselli, Lutz Hartig, Heiner Wegner, and Christian Hummert
A reliable method for the determination of type A- and B-tri-chothecenes and zearalenone in cereal-based samples is presented.

DEPARTMENTS

Peaks of Interest
Letters to the Editor
Products
Literature
Calendar
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Classified Directory
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Volume 23, Number 3, March 2005

**LC*GC March 2005 Table of Contents**

COLUMNS

COLUMN WATCH
New Chromatography Columns and Accessories at the 2005 Pittcon Conference: Part I
Ronald E. Majors
LC TROUBLESHOOTING
Split Peaks - A Case Study
John W. Dolan
GC CONNECTIONS
What's in a Method?
John V. Hinshaw
DIRECTIONS IN DISCOVERY
Chromatography Applications in Drug Discovery of Therapeutic Proteins
Paul K. Ng and Michael H. Coan

ARTICLES

Planar Chromatography Versus Column Chromatography: A Performance Comparison
Pawel K. Zarzycki, Kathrin M. Kulhanek, Roger Smith, Malgorzata A. Bartoszuk, and Henryk Lamparczyk
The authors explore the hypothesis that in particular cases, conventional planar chromatography can actually provide more effective and robust systems than column chromatography.
Characterization of Hyaluronic Acid with On-Line Differential Viscometry, Multiangle Light Scattering, and Differential Refractometry
Jason Waters and Danielle Leiske
The material characteristics of Hyaluronic acid are explored by combining a MALS instrument, a differential refractometer, and a differential viscometer with SEC and the results reported.

DEPARTMENTS

Peaks of Interest
Products
Literature
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Volume 23, Number 2, February 2005

**LC*GC February 2005 Table of Contents**

COLUMNS

COLUMN WATCH
Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part II: Changing Separation Conditions
John W. Dolan and Lloyd R. Snyder
LC TROUBLESHOOTING
Extracolumn Effects
John W. Dolan
MS - THE PRACTICAL ART
Profiles in Practice Series: Metabolism
Identification and Structural Characterization in Drug Discovery
Michael P. Balogh
MILESTONES IN CHROMATOGRAPHY
Fifty Years of GC Instrumentation
Leslie S. Ettre

ARTICLES

HPLC Analysis of Nonvolatile Analytes Using Charged Aerosol Detection
Paul H. Gamache, Ryan S. McCarthy, Scott M. Freeto, Darwin J. Asa, Matthew J. Woodcock, Katherine Laws, and Roderic O. Cole
Choice of Buffer for the Analysis of Basic Peptides in Reversed-Phase HPLC
David V. McCalley
Photodegradation and Photostability Studies of Bendroflumethiazide in Pharmaceutical Formulations and Urine Samples by Micellar Liquid Chromatography
M.J. Ruiz Ángel, M.T. Gil Agust�, J.S. Esteve Romerob, and S. Carda Brochb

DEPARTMENTS

Peaks of Interest
Pittcon Product Showcase
Literature
Calendar
Short Courses
Classified Directory
Ad Index

Volume 23, Number 1, January 2005

**LC*GC January 2005 Table of Contents**

COLUMNS

COLUMN WATCH
Preparative Chiral SFC as a Green Technology for Rapid Access to Enantiopurity in Pharmaceutical Process Research
Ronald E. Majors
LC TROUBLESHOOTING
Stay Away from the Cliffs!
John W. Dolan
GC CONNECTIONS
Flow, Pressure, and Temperature Calibration: Part II
John V. Hinshaw
VALIDATION VIEWPOINT
Analytical Method Validation: Accuracy in Quantitation
Michael Swartz and Ira Krull

ARTICLES

Elevated Temperature HPLC: Principles and Applications to Small Molecules and Biomolecules
Cuiru Zhu, David M. Goodall, and Stephen A.C. Wren
The effects of temperature on HPLC are explored, along with a critical analysis of the effects of temperature change on both peak shape and efficiency.
The Misunderstood Laboratory Solvent: Reagent Water for HPLC
Stéphane Mabic, Cecilia Regnault, and Jim Krol
The authors explore the vital importance of reagent water, the most widely used yet least characterized analytical solvent, and examine the ways that monitoring TOC levels can give chromatographers added confidence.

DEPARTMENTS

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