LC*GC
December 2010
November 2010
October 2010
September 2010
August 2010
July 2010
June 2010
May 2010
April 2010
March 2010
February 2010
January 2010
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2005
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1995 - 1996
Volume 28, Number 12, December 2010
LC*GC December 2010 Table of Contents
COLUMNS
- COLUMN WATCH
The Increasing Role of Superficially Porous Particles in HPLC
Ronald E. Majors
Ron Majors traces the development of superficially porous particle supports to the present-day versions that rival UHPLC supports.
- LC TROUBLESHOOTING
Selectivity in Reversed-Phase LC Separations, Part I: Solvent-Type Selectivity
John W. Dolan
John Dolan examines the effects of solvent-type selectivity in reversed-phase liquid chromatography.
- BIOTECHNOLOGY TODAY
Glycoproteins and Biotechnology - Recombinant Biopharmaceutical Products: Identification, Characterization, and Chemical Equivalency?
Ira S. Krull and Anurag S. Rathore
This column explores why glycoproteins have become the most common biopharmaceutical product and how they can be more fully characterized in the future.
ARTICLES
- Why System Suitability Tests Are Not a Substitute for Analytical Instrument Qualification or Calibration, Part I
R.D. McDowall
The author discusses the role of system suitability tests in the context of instrument qualification.
- Investigating Solvent Purity Using Comprehensive Gas Chromatography: A Study of Acetones
Jon H. Wahl, Cinnamon D. Bolz, and Karen L. Wahl
This work examines impurities present in a variety of acetone sources using comprehensive GC.
- 2010 Index of Authors and Subjects
LCGC North America provides its annual editorial index.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Products
- Calendar
- Classified Directory
- Ad Index
Volume 28, Number 11, November 2010
LC*GC November 2010 Table of Contents
COLUMNS
- COLUMN WATCH
A Simple Approach to Performance Optimization in HPLC and Its Application in Ultrafast Separation Development
Xiaoli Wang, Peter W. Carr, and Dwight R. Stoll
The guest authors describe an optimization procedure to achieve the highest plate count in a given analysis time.
- LC TROUBLESHOOTING
UHPLC Tips and Techniques
John W. Dolan
John Dolan presents some practical pointers to get the most out of your UHPLC system.
- GC CONNECTIONS
When Peaks Collide, Part III
John V. Hinshaw
John Hinshaw concludes his series on the effects of peak tailing.
- THE HISTORY OF CHROMATOGRAPHY
Evolution of Chromatography: One Scientist's 51-Year Journey
Thomas Beesley
Industry veteran Thomas Beesley takes readers on a tour of his experiences in chromatography.
ARTICLES
- Evaluation of QuEChERS, Cartridge SPE Cleanup, and Gas Chromatography Time-of-Flight Mass Spectrometry for the Analysis of Pesticides in Dietary Supplements
Julie Kowalski, Michelle Misselwitz, Jason Thomas, and Jack Cochran
The authors evaluate a method for detecting pesticide residues in dandelion root powder.
- Improving HPLC Separation of Polyphenols
André de Villiers, Kathithileni M. Kalili, Mareta Malan, and Jeanine Roodman
This article highlights a number of recent developments in HPLC for polyphenol analysis.
DEPARTMENTS
- Peaks of Interest
- Product Resources
- Calendar
- Classified
- Ad Index
Volume 28, Number 10, October 2010
LC*GC October 2010 Table of Contents
COLUMNS
- COLUMN WATCH
The Development and Applications of PLOT Columns in Gas-Solid Chromatography
Jaap de Zeeuw
GC expert Jaap de Zeeuw explores the historical development of PLOT columns, covers details of the most popular stationary phases, and provides practical hints.
- LC TROUBLESHOOTING
Are the Method Requirements Reasonable?
John W. Dolan
John Dolan discusses an inquiry he received from a reader complaining about the difficulty he was having meeting system suitability for an LC method that had been transferred into his lab.
- GC CONNECTIONS
When Peaks Collide, Part II
John V. Hinshaw
In Part II of this series, John Hinshaw covers the effect on tailing peak measurements when the peaks start to merge, following on from Part I, which discussed nontailing peaks.
- INNOVATIONS IN HPLC
Seeing is Believing: Detectors for HPLC
Michael Swartz
The principles and attributes of today's HPLC detectors are discussed, and the advantages and disadvantages of the various detectors are compared and contrasted.
ARTICLE
- Considerations on Switching from Helium to Hydrogen
Reginald J. Bartram and Peter Froehlich
The authors explain the operational considerations for switching from helium to hydrogen in the separations lab, as well as discussing options.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Product Resources
- Calendar
- Classified Directory
- Ad Index
Volume 28, Number 9, September 2010
LC*GC September 2010 Table of Contents
COLUMNS
- COLUMN WATCH
Highlights of HPLC 2010
Ronald E. Majors
Ron Majors presents his yearly review of the HPLC Conference.
- LC TROUBLESHOOTING
Speeding Up Gradients
Stefan Brand and John W. Dolan
Guest author Stefan Brand and John Dolan take a look at how to speed up gradient separations while avoiding common errors.
- MS-THE PRACTICAL ART
The Year of the Laser
Michael P. Balogh
On the 50th anniversary of the invention of the laser, Michael Balogh examines its importance in MS practice.
- BIOTECHNOLOGY TODAY
Popular Cell Expression Systems in the Biotechnology Industry
Thomas P. Quinn, Marianne Rivkin, Corina R. Nikoloff, and Tatyana N. Kabakova
The authors describe the most common cell-based protein expression systems and purification strategies used in the biotechnology industry.
ARTICLES
- Bare Silica Has a Future in Supercritical Fluid Chromatography
Larry T. Taylor and M. Ashraf-Khorassani
The authors compare five bonded phases with bare silica for the SFC separation of a 19-component mixture of polar analytes.
- Finding the Best Separation for Enantiomeric Mixtures
Pilar Franco and Tong Zhang
The authors describe a study of screening procedures that demonstrated a relatively small set of columns can be used routinely.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Product Resources
- Calendar
- Classified Directory
- Ad Index
Volume 28, Number 8, August 2010
LC*GC August 2010 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
The Use of Hollow Fibers in Liquid-Phase Microextraction
Limian Zhao, Hian Kee Lee, and Ronald E. Majors
In this installment, the subject of LPME is reviewed, with emphasis on the use of hollow fibers.
- LC TROUBLESHOOTING
System Contamination
John W. Dolan
Deterioration of a separation can result from many causes. This month's column looks at some techniques to isolate the problem source.
- BIOTECHNOLOGY TODAY
Biosimilars: Introduction, Concerns, and Opportunities
Anurag S. Rathore and Ira S. Krull
The authors provide an introduction to the topic of biosimilars, review the available regulatory guidance, and discuss the various scientific challenges that are present.
- HISTORY OF CHROMATOGRAPHY
Early Adventures in Chromatography: The Story of a "Recovering" Electrochemist
Peter W. Carr
Well-known industry veteran and LCGC EAB member Peter Carr looks back at the early days of the chromatography field and reflects on his own experiences.
ARTICLE
- Why Does It Take So Long To Validate a Chromatography Data System?
Robert D. McDowall
The author examines the process of validating a CDS and describes the trials along the way such as speed of validation, risk management, and so forth.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Product Resources
- Calendar
- Classified Directory
- Ad Index
Volume 28, Number 7, July 2010
LC*GC July 2010 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
QuEChERS, a Sample Preparation Technique that Is "Catching On": An Up-to-Date Interview with the Inventors
Steven J. Lehotay, Michelangelo Anastassiades, and Ronald E. Majors
The inventors of the QuEChERS technique present an update and a look at the future.
- LC TROUBLESHOOTING
Where Did That Peak Come From?
John W. Dolan
John Dolan looks at a case in which the peak doesn't seem to belong with the neighboring peaks in the chromatogram.
- GC CONNECTIONS
When Peaks Collide
John V. Hinshaw
This month's "GC Connections" addresses the effects of of two overlapping tailing peaks on peak resolution and quantitation.
- INNOVATIONS IN HPLC
Ion Chromatography: An Overview and Recent Developments
Michael Swartz
The basics of IC are reviewed and recent advances that may extend this technology to new applications are discussed.
ARTICLES
- Simultaneous Headspace-GC-FID Analysis for Methanol and Ethanol in Blood, Saliva, and Urine: Validation of Method and Comparison of Specimens
Görkem Mergen, Zeliha Kayaalti, Emrah Dural, Vugar Aliyev, Seda Kaya, Serap Yalçin, Ayşe Karakuş, and Tlin Söylemezoğlu
An HS-GC-FID method for the determination of ethanol and methanol content in biological fluids is presented.
- A Modular Approach to Pressurized Liquid Extraction with In-Cell Cleanup
Peter Haglund and Erik Spinnel
A modification of the popular PLE technique is discussed.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Products
- Literature
- Classified Directory
- Ad Index
Volume 28, Number 6, June 2010
LC*GC June 2010 Table of Contents
COLUMNS
- COLUMN WATCH
New Horizons in Reversed-Phase Chromatography
Lloyd Snyder, John Dolan, Peter Carr, and Ronald E. Majors
The coauthors discuss the various attributes of and approaches to reversed-phase chromatography.
- LC TROUBLESHOOTING
300 and Counting
John W. Dolan
John Dolan reaches a milestone with his 300th installment of "LC Troubleshooting" and takes a look back through the years.
- MS-THE PRACTICAL ART
When Electrospray Just Isn't Enough
Michael P. Balogh
John Hinshaw presents his annual review of all that was new in the field of gas chromatography at Pittcon 2010.
- BIOTECHNOLOGY TODAY
Analytical Tools for Characterization of Biotechnology Products and Processes
Ira Krull and Anurag Rathore
In the first installment of LCGC's newest column, the authors address the topic of analytical biotechnology.
ARTICLES
- Electrochemistry and LC-MS for Metabolite Generation and Identification: Tools, Technologies, and Trends
Wiebke Lohmann, Anne Baumann, and Uwe Karst
A complementary, purely instrumental method is presented that offers some interesting features compared to standard experiments. This research provides an introduction into this new approach.
- ASMS 2010 Review
Megan Evans
Associate Editor Megan Evans reviews this year's ASMS Conference, held in Salt Lake City, Utah.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Classified Directory
- Ad Index
Volume 28, Number 5, May 2010
LC*GC May 2010 Table of Contents
COLUMNS
- COLUMN WATCH
A Review of Column Developments for Supercritical Fluid Chromatography
Terry Berger and Blair Berger
The authors review the requirements for columns specifically designed and manufactured for SFC.
- LC TROUBLESHOOTING
Too Much Scrutiny
John W. Dolan
In recent conversations at Pittcon 2010, it became clear to the author that sometimes we get too focused on the details of the method without backing up and determining how they fit into the big picture.
- GC CONNECTIONS
New Gas Chromatography Products at Pittcon 2010
John V. Hinshaw
John Hinshaw presents his annual review of all that was new in the field of gas chromatography at Pittcon 2010.
- INNOVATIONS IN HPLC
HPLC Systems and Components Introduced at Pittcon 2010: A Brief Review
Michael Swartz
Michael Swartz presents his first annual review of HPLC systems and accessories at Pittcon 2010.
ARTICLE
- Real-Time Profiling of Volatile Malt Aldehydes Using Selected Ion Flow Tube Mass Spectrometry
Jessika De Clippeleer, Filip Van Opstaele, Joeri Vercammen, Gregory J. Francis, Luc De Cooman, and Guido Aerts
The authors use headspace SIFT-MS to target and identify volatiles in various malt aldehydes. The specificity and speed are compared to current methodology.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Products
- Literature
- Classified Directory
- Ad Index
Volume 28, Number 4, April 2010
LC*GC April 2010 Table of Contents
COLUMNS
- COLUMN WATCH
New Chromatography Columns and Accessories at Pittcon 2009: Part II
Ronald E. Majors
Ron Majors presents the second part of his yearly review of all that was new and innovative at Pittcon 2010, held this year in Orlando, Florida.
- LC TROUBLESHOOTING
Getting the Most Out of Calibration Standards
Jennifer Birchett and John W. Dolan
In this month's "LC Troubleshooting," the authors look at another use of calibration standards: to monitor the quality of the data, with a goal toward helping scientists troubleshoot instrument problems.
- MS-THE PRACTICAL ART
Could This Be Your MS System of the Future?
Michael P. Balogh
With the advances in LC-MS in recent years, is there another practical option on the horizon for those who need the specificity of MS and can justify the narrow definition of a purpose-built system?
ARTICLE
- Simultaneous Determination of Synthetic Pharmaceutical Peptides and the Acetate Counterion by Mixed-Mode Weak-Anion-Exchange Chromatography
Deanna C. Hurum, Brian M. De Borba, and Jeffrey S. Rohrer
Oxytocin acetate samples were analyzed successfully for both the peptide and acetate with a single method using a mixedmode weak-anion-exchange column, demonstrating the applicability of this methodology to peptide analysis.
DEPARTMENTS
- Peaks of Interest
- Products
- Literature
- Classified Directory
- Ad Index
Volume 28, Number 3, March 2010
LC*GC March 2010 Table of Contents
COLUMNS
- COLUMN WATCH
New Chromatography Columns and Accessories at Pittcon 2009: Part I
Ronald E. Majors
Ron Majors presents his yearly review of all that was new and innovative at Pittcon 2010, held this year in Orlando, Florida.
- LC TROUBLESHOOTING
Enhancing Signal-to-Noise
John W. Dolan
In order to improve the LLOQ or LOD, or to increase precision in any case where S/N degrades precision, an increase in S/N will be beneficial. Here, John Dolan focuses on improvements in signal-to-noise.
- GC CONNECTIONS
Spring Cleaning
John V. Hinshaw
In this month's "GC Connections," John Hinshaw addresses GC components that can become contaminated, including symptoms, causes, and cleaning procedures.
ARTICLES
- The 2010 LCGC Pittcon Awards
David Walsh
The winners of LCGC's third annual awards were announced at Pittcon 2010.
- A Global Approach to HPLC Column Selection Using Reversed Phase and HILIC Modes: What to Try When C18 Doesn't Work
Merlin K. L. Bicking and Richard A. Henry
Experimental data are presented to help understand HPLC column retention in a way that should make column selection and method development proceed faster when initial results with C18 columns are disappointing.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Products
- Literature
- Classified Directory
- Ad Index
Volume 28, Number 2, February 2010
LC*GC February 2010 Table of Contents
COLUMNS
- SAMPLE PREP PERSPECTIVES
Electromembrane Extraction: The Use of Electrical Potential for Isolation of Charged Substances from Biological Matrices
Astrid Gjelstad
This minireview of electromembrane extraction demonstrates how the combination of an electroextraction with hollow-fiber liquid-phase microextraction can lead to a selective, rapid new method.
- LC TROUBLESHOOTING
Column Diameter, Linear Velocity, and Column Efficiency
John W. Dolan
John Dolan examines some questions he recently received concerning the changes that took place in the 2009 edition of the United States Pharmacopoeia.
- MS-THE PRACTICAL ART
The Nature and Utility of Mass Spectra
Michael P. Balogh
This month's installment addresses some elementary aspects of the mass spectrum, the tools employed by the experienced practitioner, and advances being made into identifying unknowns.
- HISTORY OF CHROMATOGRAPHY
A History of Gas Chromatography: My Early Experiences
Harold McNair
Industry veteran and 2009 LCGC Lifetime Achievement Award winner Harold McNair discusses the history of temperature programming in the field of GC.
ARTICLE
- Multiple Dimensions of Separations: SPME with GCxGC and GCxGC-TOF-MS
Nicholas H. Snow, Jerianne Sinex, and Mandelle Danser
The combination of SPME with GCxGC and GCxGC-TOF-MS is discussed using mixtures of solvents as example analytes.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Products
- Literature
- Classified Directory
- Ad Index
Volume 28, Number 1, January 2010
LC*GC January 2010 Table of Contents
COLUMNS
- COLUMN WATCH
Hydrogen as a Carrier Gas for GC and GC-MS
James Heseltine
Guest columnist James Heseltine discusses hydrogen as an effective replacement for helium in many GC and GC-MS applications.
- LC TROUBLESHOOTING
Too Little or Too Much
John W. Dolan
John Dolan discusses an all too common problem: What to do when a peak is too small or too large.
- GC CONNECTIONS
Upgrading Gas Chromatography
John V. Hinshaw
This month's "GC Connections" explores the issues that can arise when deciding to upgrade or replace GC technologies.
- INNOVATIONS IN HPLC
HPLC: Continuing to Answer Challenges to Food and Drug Safety
Michael Swartz
Three analysis challenges have been receiving a great deal of attention: the water we drink, the food we eat, and the drugs we take. Michael Swartz discusses the use of HPLC to address these threats to health and safety.
ARTICLE
- A Generic Headspace GC Method for Residual Solvents in Pharmaceuticals: Benefits, Rationale, and Adaptations for New Chemical Entities
Lulu Dai, Amanda C. Quiroga, Ke Zhang, Heather B. Runes, Derrick T. Yazzie, Kavita Mistry, Nik P. Chetwyn, and Michael W. Dong
The authors describe a generic static headspace gas
chromatographic (HSGC) method for determining common residual solvents in pharmaceuticals.
DEPARTMENTS
- From the Editor
- Peaks of Interest
- Products
- Literature
- Classified Directory
- Ad Index